| Step 1: Upload data | |
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Group
Supported File Formats: .gpr, .txt(tab delimited), .csv, .xlsx, .xls COVID-19 patient Healthy control |
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Group
Press the "Confirm" button to set the groups for comparison. |
Group
Press the "Confirm" button to set the groups for comparison. |
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Step 2: Specify control spots |
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Control spots identified by protein IDs/Names:
Control spots identified by # of repeated protein names >= |
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| Step 3: Data pre-processing | |
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3.1 Procat:
Yes No |
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3.2 Select signal statistics:
By column name: / |
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3.3 Select the data range normalization algorithm:
Median normalization Mean normalization Quantile normalization z-score normalization Min-Max normalization Robust normalization None |
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| Step 4: Filtering by quality control & differential analyses | |
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Experimental Group
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Control Group
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4.1 Within-Group Quality Control filters for each protein:
At least sample with raw intensity CV of sample normalized intensities <= % Average intensity of exprimental group [Average of all proteins] * [SD of all proteins] |
4.1 Within-Group Quality Control filters for each protein:
At least sample with raw intensity CV of sample normalized intensities <= % Average intensity of control group [Average of all proteins] * [SD of all proteins] |
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4.2 Between-Group Differential Analysis filters for each protein:
|Difference Average of exprimental group and control group| ≥ SD of all spots Average of exprimental groups/Average of control groups ≥ or Average of exprimental groups/Average of control groups ≤ Difference Average of exprimental group and control group (Experimental group - Control group) ≥ [Difference average of all proteins] + * [Difference SD of all proteins] or ≤ [Difference average of all proteins] - * [Difference SD of all proteins] Statistical Tests (Two-sided):
K-test
U-test
T-test
Multiple hypotheses testing correction: ; Threshold: |
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