| Step 1: Upload data | |
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Group
Supported File Formats: .gpr, .txt(tab delimited), .csv, .xlsx, .xls |
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Step 2: Specify control spots |
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Control spots identified by protein IDs/Names:
Control spots identified by # of repeated protein names >= |
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| Step 3: Data pre-processing | |
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3.1 Select signal statistics:
By column name: / |
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3.2 Select the data range normalization algorithm:
Median normalization Mean normalization Quantile normalization z-score normalization Min-Max normalization Robust normalization None |
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| Step 4: Filtering by quality control & differential analyses | |
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4.1 Within-Group Quality Control filters for each protein:
At least sample with raw intensity CV of sample normalized intensities <= % Average intensity [Average of all proteins] * [SD of all proteins] |
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Statistical Tests (Two-sided):
ANOVA
Kruskal-Wallis H-test
Multiple hypotheses testing correction: ; Threshold: |
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