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The EST data can be searched and retrieved using the following search criteria: *

EST Annotator: List all EST Information, Annotation, Pathway and Go Term. And search EST libraries
by multiple keywords at the same time.

This function performs a text search among the pre-computed BLAST results against NR for all assembly products, with an
E-value cutoff of 1e-5. Type in the name of the gene you are interested in, keeping in mind that the description of NCBI accessions
references genes in different ways. For example, searching for “Annotation: protein, Species: Oryza” will yield all BLAST results as
the following figure 1-a, figure 1-b, figure 1-c.

figure 1-a. Select Library Name and Click ’View/Search’ button, will list all EST information.

figure 1-b. Search EST libraries by multiple keywords at the same time. (Keyword: Annotation: protein  Species: Oryza)

figure 1-c. Snapshots of search results

TC (tentative contig) Annotator: List all TC Information, Annotation, Pathway and Go Term. And search EST libraries by multiple keywords at the same time, shows as the figure 2 below.

figure 2. Search EST libraries by multiple keywords at the same time.

Gene Ontology: Classification of TCs by GO vocabularies, shows as the figure 3 below.

figure 3. Snapshots of search results

Metabolic Pathways(Tree view): Association of TCs with metabolic and signaling pathways, shows as the figure 4 below.

figure 4 Snapshots of analytic results

Metabolic Pathways(List view): Association of TCs with metabolic and signaling pathways, shows as the figure 5-a, figure 5-b below.


figure 5-a Snapshots of search results


figure 5-b Snapshots of search results

Search by BLAST (Phalaenopsis Transcriptome)
Search by BLAST (Orchidaceae Floral Transcriptome)

The user can paste sequence to BLAST against EST data sets from the orchids. Alignment results could be viewed and the hit sequence could be retrieved.

figure 6 Snapshots of BLAST results

Multiple Gene Network Analysis(Phalaenopsis Transcriptome)

figure 7 Gene Network Search

Multiple Gene Network Analysis(Phalaenopsis Transcriptome)

figure 8 Multiple Gene Network Analysis