CLASH Analyst: A web server to identify
in vivo
RNA-RNA interactions from CLASH sequencing data
Input
Results
Tutorial
Contact
Upload file
Load example files
Reanalyze old data?
CLASH read file
Sample
Upload here
Regulatory RNA file
Sample
Select data
Select data
Upload by yourself
Human
Human/miRNA
C.elegans
C.elegans/piRNA
C.elegans/miRNA
Upload here
Target RNA file
Sample
Select data
Select data
Upload by yourself
Human
Human/mRNA
C.elegans
C.elegans/mRNA
Upload here
Job ID
CLASH read preprocessing
FASTQ file already trimmed?
Barcode sequence (optional)
Trimming tool
trim_galore
flexbar
fastx
3' adapter sequence
(optional)
Minimum required read length ≥
Maximum required read length ≤
Phred score (Q score) ≥
Identify Chimeras
Algorithm
CLAN
Hyb
piRTarBase
Minimum length for each RNA fragment ≥
Overlap between two RNA fragments ≤
Hits per fragment ≤
Fragment selection threshold ≤
Overlap/Gap between two RNA fragments ≤
Hits per read ≤
Mismatch of aligning to regulatory RNA ≤
Mismatch of aligning to target RNA ≤
Remaining sequence length ≥
Hits per read ≤
User information (optional)
Provide an E-mail address to be notified when your results are ready as analysis can take a long time
(strongly recommend)
Submit
Addictional information
For an easy-to-follow LAguide:
Example Quick Start Guide
If you cannot successfully run the example file in this server, try another server:
https://cosbi7.ee.ncku.edu.tw/CLASHanalyst/
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